5 Other toolkits¶
This section intentionally avoids duplicating commands already documented in the workflow. It summarizes only auxiliary utilities and where to find them.
Utility groups¶
| Group | Commands | Purpose |
|---|---|---|
| Ribo-utils not covered above | rpf_Shuffle, rpf_Shift, rpf_Bam2bw, rpf_Retrieve , rpf_Geneplot |
shuffled controls, translation frame shift, signal track generation, retrieve rpf density, gene plotting |
| Ribo-utils merge helpers | merge_length, merge_saturation, merge_digestion, merge_offset, merge_offset_detail , merge_dst_list, merge_period, merge_metagene, merge_coverage, merge_quant, merge_pausing, merge_occupancy, merge_cdt, merge_cst, merge_odd_ratio |
length distribution, gene saturation, rpf digestion, rpf offset, rpf offset details, rpf density file, 3nt periodicity, rpf metagene, rpf coverage, rpf quant, translation pausing score, translation occupancy, translation codon decoding time, translation codon selection time, translation codon odd ratio |
| FASTA helpers | fa_gc_sum, fa_len_flt, fa_len_sum, fa_split, line_feed, nt2aa, rand_seq, retrieve_seq, revs |
sequence preprocessing and extraction |
| FASTQ helpers | fq_len_flt, fq_len_sum, fq_length, fq_split, fq_trim, fq2fa, fq2txt, phred_quality, simulate_fastq |
read preprocessing and simulation |
| bedGraph helpers | bg2meta, rpm_smooth |
signal processing |
| Bowtie/RSEM helpers | merge_bwt_log, merge_rsem |
mapping and quantification summary |
| RiboCode/RiboTISH helpers | ribocode_bed_format, ribotish_format |
external ORF-tool output formatting |
| Unix helpers | dos2unix |
line-ending conversion |
| oligo helpers | get_overlap_seq, get_tissue_freq, get_win_seq |
sequence-window and oligo-related processing |